Microscopy stains and dyes

manalokos · Post by **manalokos** » Mon Jan 24, 2011 11:11 pm

Hello!

Has anyone tried stains or dyes other than methylene blue to make DCG
more sensitive to Green lasers? Something like Carmine would do the trick!
http://en.wikipedia.org/wiki/Carmine

Does the dye need to have a very special chemical reaction to work?
Sorry for the dumb questions...

Best regards
Filipe

holorefugee · Post by **holorefugee** » Tue Jan 25, 2011 1:20 pm

If you search the archive there are a couple of other dye's that Jeff Blythe has used. The may be in the wiki as well.

info.crew · Post by **info.crew** » Tue Mar 01, 2011 10:57 am

Hello!
I tried the Eosine and Rhodamines (6G and B), but i noticed the coagulation of the eosine. R6G has best sensitivity, B is not so sensitive. But I did not make any in-depth experiments.

a_k · Post by **a_k** » Wed Mar 02, 2011 3:03 pm

You can find some dyes in Jeffs patent Nr EP2232263:

http://holoforum.org/data/doc/patents/B ... ic_DCG.pdf