vasimv wrote: ↑
Sun Sep 01, 2019 5:16 pm
Worst problem is emulsion tends to peel off (like in upper-left corner - dots in areas where emulsion got removed) after that.
Around 1999 or so, Jeff Blyth published his silver halide diffusion method (which I think he updated and improved since then). I think in one of his papers (about that time or later), he detailed his glass preparation method. The diffusion method required that the gelatin adhere strongly to the glass. This involved the use of the 3-aminopropyltriethoxysilane which I mentioned earlier. I use this method, and it works extremely well. It's part of my routine, and I don't omit it. Gelatin does not come off the glass when using it. I believe another name for the same thing is gamma-aminopropyltriethoxysilane. This stuff does not like water, either water vapor in the air, or any water present in the IPA used to dilute it. The IPA can have water content, but apparently this doesn't store well (the silane/IPA mixture). I guess this is the reason why it's recommended to mix it fresh, each day. All you need to do is mix the silane/IPA, rub it on the glass, and let it sit in the open air for a couple of hours (more or less).
The only problem is in finding the silane. Ebay has that rather costly large volume in a bottle ($200). But I think this same silane might be available from other sources, if some searching is done. I believe microscopists use it for some types of slide preparation, and microscope equipment suppliers might have smaller volumes at less cost (I seem to recall seeing this many years ago).
This is available from a glass-working supplier in the US:
https://www.hisglassworks.com/bond-and- ... -html.html
I'm don't think this is the best way to buy the silane, though, because it's pre-mixed, and any bit of moisture in the solvent used by the supplier will likely affect shelf life. It may be worth a try however.
Thanks for the info on the collagen.